![]() ![]() There are other components that show remedial properties, but curcumin is the only component that shows microbial activity. When I re-read my notes on curcumin, I realized that curcumin is the active ingredient in the root curcuma longa and turmeric. Since the samples are extremely small, different concentrations does not seem feasible. From what you said and from what I've read, paper chromatography is not an effective method to test its effects on micro organisms. I talked to my mentor about the isolation of curcumin from turmeric, and she said paper chromatography. What will you measure, and how will you measure it? Growth rate? Carbon dioxide production (especially with yeast)? You need to think what will be your dependent variable. If you do, buy several packages and make sure they are all from the same manufacturing batch. You may want to look into using baker's dry yeast, available at many supermarkets. You will probably want to test several doses on your model organism. Try visiting nutritional supplement stores in your locality. Search for these terms on google:įor example, here is one source (I know nothing about them, and this does not constitute a recommendation to buy from them): It would be laborious and distracting for you to purify it yourself. It is very soluble in ethanol, apparently less so in water. If you purify curcumin yourself, you will use paper chromatography or thin layer chromatography (TLC) to see how pure it is, usually by comparing to a standard of known purity.Ĭurcumin is obtained by crystallization. The amounts used are miniscule, and will not be useful to purify any useful amount to use as a treatment. ![]() The purpose of the controls is to tease out the effects of all of the treatments you are using, and leave no doubt as to what component is causing what effect.Īs to purifying curcumin, you cannot do it on a useful scale with paper chromatography, which is a method to detect the presence of substances in a mixture. I don't think the terminology of positive or negative control is important in your case. Yoyu may even have an additional control group to which you add 5 ml of pure water to control for the effects of the alcohol. This means that if you needed to dissolve the curcumin in 70% alcohol in water and applied 5 ml of that solution, you would apply 5 ml of 70% alcohol in water without the curcumin to the control group. The one without is to be treated with the same materials as that of curcumin, exept without curcumin. It sounds as if your experiment may be as simple as one test group with curcumin, and one without. When the results show the separated substances in a certain order from let's say substances 1-4, if I conduct the same test, will substance 2, for example, always be shown in the same location? Would I be able to control the order in which the substances separate?Īs to how to design your experiment, please read some posts I have made in the "Plant Nutrition" thread here in the Life, Earth, and Social Sciences forum. I have researched on paper thin chromatography, and it seems like the best way to go in terms of isolating curcumin. Curcumin is the active ingredient in curcuma longa, but from what I have research, there are other components of the plant that also show some healing properties. My third question is to do with isolating curcumin, which comes from curcuma longa. If I could be provided with suggestions or information or websites that can help me make my decision, I would be most grateful. I have many choices, but I am confused on what to choose. She suggested brine shrimp and E.Coli as other possible organisms to conduct the test on. If curcumin inhibits growth of paramecium, then I can predict it can for other closely related protists. The reason I initially chose paramecium is because it can be used as a model in my experiment. I began this year thinking about testing the effect of curcumin on paramecium, but my teacher said it would be really hard to acquire 100 +/- samples of this protist because it is so hard to 'catch' if that is the right word. I am most definitely conducting my experiment at school, so I cannot use any harmful micro organisms. It would also be really great if an example could be given as well.įor my second question, this regards the organism I have to choose. I have tried reading up on what positive and negative controls are, but haven't gotten a clear answer. My teacher was talking about what I could do, and she mentioned positive and negative controls. However this is not relevant to my first question). I have started my project this year, and I am testing the effects of curcumin on an organism (what organism, I haven't decided. ![]()
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